HONOURABLE MENTION

#### 5 second rule

we are researching/testing to see if the five second rule is true .

### Hypothesis

Caras prediction

predict it will be unsanitary to eat food off the floor. I don't think it will do much harm though if you eat it but I also don't think you should not eat food off a floor in a public place because then you risk eating animal

fecal mature.GROSS.if you search the question can you eat food off the floor it says if you pick it up within 5 seconds but we wanna take that to the test by experimenting and research our project is to figure out if it is safe to eat food off the floor however we want to see if other surfaces are worse to eat off or not  We are testing this out on different surfaces and see if it is okay I think a kitchen floor will be fine because we do not have an animal however I do think the one when dropped on the school floor will not be very good for you to eat because so many things have touched that floor and I think the same about the phone that gets touched a lot and not a lot of people wash their hands before using their phone so you may have touched something before using your phone and know that is on your phone. Carpet collects a ton of dust and germs also bacteria so I do think this one will collect germs and is probably not great to eat and you also need to think about what bugs and pesticides are on that carpet or even dead ones also what chemicals are in the fibers of the carpet.

Yasnas prediction

I think that it isn’t safe to eat food off the floor even before 5 seconds because the floor might be really dirty and the germs can still stick to the apple/food. so no I don't think it's safe to eat food off the floor. We searched up can you eat food off the floor and it said if it's within 5 seconds it is safe. but we wanna test that and also research it to see if it's actually true. we dropped a piece of apple on 4 different surfaces. I think the kitchen one probably will be fine. but I don't think it's really ok to eat food if it falls on your phone carpet or school floor. The carpet might be hiding a lot of secrets and having a lot of tiny bugs germs and bacteria in it. the school floor is probably really dirty because everyone steps there and you don't really know what's been going on. the phone is probably really dirty too because a lot of people probably touched it or you might have touched it after doing something and it's probably really dirty.

### Research

is the 5-second rule true or a myth? lots of sources said it was true you can eat the food of the floor because in those 5 seconds it has not collected any germs as opposed to leaving it for 20 seconds these websites included..www.webmc.com,en.Wikipedia.org,melrobbins.com,www.Healthline.org.But some sources said that even though its a well-known rule doesn't mean its true and that in those 5 seconds it can collect small germs called bacillus, micrococcus, pseudomonas, and staphylococcus and if your really unlucky salmonella or fecal matter these websites include...kidshealth.org,www.foodsafety.ca,healthblog.uofmhealth.org and www.bbc.com.But one specific site caught our eye when it talked about both sides saying some dry food like a cracker may not collect as much bacteria as per sa a bagel with cream cheese face down but she also said sometimes it's not all about the food it's more from the timing about more. This is by scientificamerica.com and this laddie's so-called name is everyday einstein. She also has a podcast that goes into more detail.    How we eat food is by breaking it into small pieces with our teeth and then start the digestion of sugars with chemicals called enzymes our tongue pushes the food to the back of our mouth where we then swallow it and it travels down the esophagus to our stomach. When we swallow bacteria, antibiotics are how we kill then .antibiotifcs kill good and bad bacteria probiotics help replenish good bacteria they may also turn into a yeast overgrowth which is also known as chronic candidiasis that can lead to several severe symptoms that include gas, bloating, mood swings, and lapses of attention. This research was found on www. mitchellmedicalgroup.com, www. Healthline .com, www. gikids.org

### Variables

For this project we conducted four different experiments to help us get a more accurate germ analysis .starting with #1 we tested a kitchen floor because a lot of people eat of their own floor because they do not think it is dirty but by this part of the experiment we will take that thought to the test.#2 for our second experiment we did a school floor because lots of people touch that floor with the shoes they just went wherever with and know that is on the floor and also on the food you dropped.#3 we decided on doing a carpet because carpet conducts a ton of dust and bacteria and most likely chemicals from the making process of the carpet .#4 last but not least we wanted to tackle a more unknown experiment which is food on your phone which also kind of related to touching your phone before eating also we know a phone can be really germ-infested.

### Procedure

after we found an idea for our science project we bought the supplies we needed ( agar powder and Petri dishes ) and started getting to work. we first needed to make the agar gel and apply it to the petri dish. To make the agar gel we needed to boil some water and mix it with the powder.  We left the water so it could cool down a bit and then mixed it with the agar powder. after that, we poured the gel we just made in the petri dish and left it so it can harden ( the gel took about 2 days to harden ). After the gel was hardened we dropped a piece of apple on four different surfaces and left it for 4 to 5 ish seconds.  The 4 surfaces we dropped a piece of apple on were the school floor, kitchen floor, back of a phone, and carpet. After we dropped the apple on 4 different surfaces we swabbed it with a q-tip and rubbed the q-tip on the Petri dishes. after a few days, we also added a small piece of apple to the petri dish so it might help the mold grow.

### Observations

with our observations, we were excited to learn and see some different mold growth between the four Petri dishes all of which are apples. the one from the school had mold on top and was turning black and another section of the petri dish had a black spot. But with the one from a kitchen floor, it was also turning a little black but it had some almost like a virus mold growing on it there were multiple little lines sticking out with little circles at the end of the mold. Opposed to the other two which weren't having the slightest signs of mold these two had just aged a little bit but hadn't grown anything. We also observed the length of time it took for any mold to grow on any of the four so we definitely found that very interesting. And maybe this was because the last two didn't touch any germs but this would also go against our original hypothesis but science is all about learning and we were definitely gonna challenge this no mold growing thing by a lot of research to figure out why that happened and if somewhere in the process we messed up.

### Analysis

when doing the 5-second rule we broke it down into small sections the very first part was to conduct our experiment. The things we needed were Petri dishes and agar gel. To make the actual agar gel we had to boil some water, pour it into the Petri dishes, and wait about 3-5 min to harden. Agar gel is like a substance that creates a surface to help create the right environment for mold to grow. The first place we dropped a piece of apple on was the school floor. then when we got back home we dropped a piece of apple on the kitchen floor, on the carpet, and on a phone. before we moved on to the next section we swabbed them and added a sliver of apple into each.

Next, we moved on to research, during the research process we learned lots of stuff about the five-second rule is a myth, and websites saying it was true when also learned the process of mold growth and found lots of info on the different types of food and their mold process.

Step 3 was to be keeping up with the scientific method and fill out all of those sections and keep a logbook

### Conclusion

with our experiment and research, we found that with eating food off the floor it is very important that you check that you're not eating something that has came in contact with a ground that an animal has been on because that is when some major germs can come into play and enter your immune system and that is one of the ways you could get sick. We did find with our apple experiment you could eat off of those four surfaces that we tested because we weren't seeing anything growing that could be bad for your body. But if you are big on hygiene you still may not want to eat something moist on the floor like a bagel with cream cheese because that can collect more germs because of the moisture. And our conclusion is that eating food that has been on the floor for 5 seconds can be consumed not doing much harm to your body but we still wouldn't recommend it because it is kinda gross.

our science fair project was about if the five-second rule true, and this is the answer...

The Five-second can be technically true or false because germs are very sticky creatures so they are able to stick to your food as soon as it touches the floor. This really depends mostly on the food that you are using/eating AND the surface you dropped it on. Some surfaces might not be as clean or dirty as others so it really depends on the surface you dropped your food on. The food that you are using/eating might be dry, sticky, moist, or any other texture. The foods that are dry like bread or crackers might be safer to eat than moist foods like the inside of most fruits (for example inside of an orange or kiwi) and maybe a bagel with cream cheese on it, & because these foods are more sticky, it helps the germs, even more, to stick to your food. So the five-second rule is technically not really true for some foods but it is not gonna do much harm unless it comes in contact with an animal.

### Application

I think this relates to the real world because

we want to keep our bodies safe and healthy and clean. Also, lots of people commonly do this they eat of the floor. We did a little experiment to see how many people in our class eat of the floor.75.86 percent of our class has eaten of the floor and that is why we wanted to do this project because we were curious to see if that is safe.

### Sources Of Error

Throughout this process, we noticed that the mold was only growing on two of the Petri dishes and not the other two. The Petri dishes that belong to the school floor and the kitchen floor grew a little bit of mold but the other two that were from the carpet and phone didn't change a single bit since the first day. We think it's probably because those two surfaces weren't as dirty as the other two, so that might be why the mold didn't grow on them, but we might have done something wrong through the process because we were very surprised that mold didn't grow because we weren't even seeing signs of it aging.

### Citations

these are the websites we used for our research and to compare opinions we found these as good reffrences.

www.webmc.com, en.Wikipedia.org , melrobbins.com, www.Healthline.org

kidshealth.org , www.foodsafety.ca, healthblog.uofmhealth.org and www.bbc.com

### Acknowledgement

www.webmc.com, en.Wikipedia.org, melrobbins.com, www.Healthline.org, www.foodsafety.ca, healthblog.uofmhealth.org, and www.bbc.com These are the websites we used to find a lot of our information and some other things from youtube. We also used a few more websites for some smaller pieces of information as well.

our science fair project was about if the five-second rule true, and this is the answer...

The Five-second rule can be technically true or false because germs are very sticky creatures so they are able to stick to your food as soon as it touches the floor. This really depends mostly on the food that you are using/eating AND the surface you dropped it on. Some surfaces might not be as clean or dirty as others so it really depends on the surface you dropped your food on. The food that you are using/eating might be dry, sticky, moist, or any other texture. The foods that are dry like bread or crackers might be safer to eat than moist foods like the inside of most fruits (for example inside of an orange or kiwi) and maybe a bagel with cream cheese on it, & because these foods are more sticky, it helps the germs, even more, to stick to your food. So the five-second rule is technically not really true because germs can still stick to your food in either way if it's a piece of bread or a piece of apple, and you don't need to count 5 seconds to pick it up because when you get to 1 a lot of germs are already on your food.